3. Gene Delivery and Expression Series Gene Delivery and Expression (1)
Division of Medical and Biological Chemistry, Graduate School of Medicine, Nippon Medical School
The delivery of genes into cells is a basic procedure in molecular biology. The induction of genes into prokaryotic cells is referred to as transformation, which is a basic method in gene cloning, whereas that into eukaryotic cells is called transfection. Several transfection methods have been reported: physical, chemical, and biological. In this technical note, a transfection method using cationic lipids is described for studies of gene function and the expression of mutant proteins.
ϊγεγο 2011; 7(2), 92-96
transformation, transfection, vector, cationic lipids, luciferase
Hideo Orimo, MD, PhD, Division of Metabolism and Nutrition, Department of Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku Tokyo 113-8602, Japan